Journal: mBio

Article Title: Cryptococcus neoforman s rapidly invades the murine brain by sequential breaching of airway and endothelial tissues barriers, followed by engulfment by microglia

doi: 10.1128/mbio.03078-23

Figure Lengend Snippet: List of antibodies and dyes used

Article Snippet: AF647 – Podocalyxin , 192703 , FAB1556R , Bio-techne , 22.5 µg/mL , Endothelium.

Techniques: Staining, Marker

Journal: Oncotarget

Article Title: CXCR6-CXCL16 axis promotes prostate cancer by mediating cytoskeleton rearrangement via Ezrin activation and α v β 3 integrin clustering

doi: 10.18632/oncotarget.6944

Figure Lengend Snippet: PC3 and LNCaP cells cultured on cover slips were treated with 100ng/ml CXCL16 for 5 min with or without Calphostin C (100 nM) or Wortmannin (10 μM) pretreatment. Cells were stained with Rhodamine Phallodin and Alexa Fluor 488 conjugated Mouse anti-Ezrin (pY353) antibody. Images were captured using Olympus FluoView FV1000 confocal microscope with 60X oil immersion objective. Scale bar represents 20μm.

Article Snippet: After washing with wash buffer (Cytoskeleton Inc. Denver), cells were incubated for 40 minutes with 100nM Rhodamine Phallodin and 20μl Alexa Fluor 488 conjugated Mouse anti-Ezrin (pY353) (BD Biosciences).

Techniques: Cell Culture, Staining, Microscopy

Journal: STAR Protocols

Article Title: Protocol for measuring killing capacity and intracellular cytokine production of human HIV antigen-specific CD8 T cells using flow cytometry

doi: 10.1016/j.xpro.2024.103231

Figure Lengend Snippet:

Article Snippet: BD Pharmingen Alexa Fluor 700 mouse anti-human TNF, dilution: 1:160 , BD Biosciences , 561023.

Techniques: Blocking Assay, Recombinant, Staining, Flow Cytometry, Infection, Cell Isolation, Software